Aldolase activity of serum albumins.
نویسندگان
چکیده
Bovine and human serum albumins catalyze the aldol reaction of aromatic aldehyedes and acetone, with saturation kinetics and moderate and opposite enantioselectivity. The reaction occurs at the binding site in domain IIa, and is inhibited by warfarin. Kinetic data are consistent with an enamine mechanism. The activity is conserved in a 103 aminoacid peptide derived from the albumin sequence.
منابع مشابه
Coating of Iron Oxide Nanoparticles with Human and Bovine Serum Albumins: A Thermodynamic Approach
In this research, the Magnetite nanoparticles (Fe304) were prepared by coprecipitation of Fe3+ andFE solution in alkaline medium. Two kinds of surfactants, cetyl tri methyl ammonium bromide(CTAB) and cetyl pyridinum chloride (CPC) were used in the synthesis. Fe304 Nanoparticles werecoated with human serum albumin (HSA) and bovine serum albumin (BSA). Characteristics ofcoated magnetic nanopartic...
متن کاملDiagnosis of Allergy to Mammals and Fish: Cross-Reactive vs. Specific Markers
PURPOSE OF REVIEW Allergen extracts are still widely used in allergy diagnosis as they are regarded as sensitive screening tools despite the fact that they may lack some minor allergens. Another drawback of extracts is their low specificity, which is due to the presence of cross-reactive allergens. Progress in allergen identification has disclosed a number of allergenic molecules of homologous ...
متن کاملEnhanced aldolase activity and glycolysis as short term effects of hydrogen cyanide for the release of dormancy in walnut kernels
Seed dormancy removal by cold stratification is accompanied by the development of gluconeogenic competence. Although hydrogen cyanide can stimulate the germination of many herbaceous dormant seeds and increase gluconeogenesis in long term, its short-term effects on sugar metabolism require further investigation. Accordingly, an experiment in the form of complete randomized design was carried ou...
متن کاملThe Immune Response of Rabbits Tolerant to Bovine Serum Albumin to the Injection of Other Heterologous Serum Albumins
Immunological tolerance produced in rabbits by neonatal injections of BSA can be terminated by a series of injections of certain heterologous serum albumins which cross-react with BSA. Injections of albumins distantly related to BSA were more effective in terminating the tolerant state than injections of albumins closely related to BSA. It was concluded from results obtained with several hetero...
متن کاملCharacterization of Some Physico-chemical Properties and Interactions of Human and Bovine Serum Albumins with Mitomycin C
Physico-chemical properties, molecular interactions and thermodynamic stability of human and bovine serum albumins in complexes with mitomycin C were investigated. The pH and temperature influence on serum albumins properties and the binding mechanism of mitomycin C to these proteins have been monitored by spectrometry and spectrofluorimetry methods.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Organic & biomolecular chemistry
دوره 9 12 شماره
صفحات -
تاریخ انتشار 2011